{"id":2290,"date":"2026-01-28T17:48:30","date_gmt":"2026-01-28T16:48:30","guid":{"rendered":"https:\/\/salvak.com.co\/aplicaciones\/pcr-qpcr-rt-pcr\/"},"modified":"2026-04-27T23:06:01","modified_gmt":"2026-04-27T21:06:01","slug":"pcr-qpcr-rt-pcr","status":"publish","type":"aplicaciones","link":"https:\/\/salvak.com.co\/en\/applications\/pcr-qpcr-rt-pcr\/","title":{"rendered":"PCR\/ qPCR\/RT-PCR"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\"><strong>PCR:<\/strong><\/h4>\n\n<p>Polymerase Chain Reaction (PCR) is a fundamental molecular biology technique that allows the amplification, that is, the generation of millions of copies of a specific DNA fragment from a very small amount of sample. This amplification makes it possible to study, detect, or analyze specific regions of genetic material that would otherwise be too small to work with. <br\/>Thanks to its precision and sensitivity, PCR is used in areas such as molecular diagnostics, forensic analysis, biomedical research, genetic studies, food safety, epidemiological surveillance, and more.<\/p>\n\n<h4 class=\"wp-block-heading\"><strong>qPCR (real-time PCR):<\/strong><\/h4>\n\n<p>qPCR, or real-time PCR, is a variation of traditional PCR that allows the amount of DNA amplified to be detected and quantified while the reaction is taking place, not only at the end.<br\/>Unlike conventional PCR, qPCR uses:<br\/>&#8211; Fluorescent dyes or specific probes that emit light proportional to the amount of DNA generated.<br\/>&#8211; Specialized equipment that measures this fluorescence in real time.<\/p>\n\n<h4 class=\"wp-block-heading\"><strong>RT-PCR (reverse transcription PCR):<\/strong><\/h4>\n\n<p>RT-PCR combines two processes into one:<br\/>&#8211; Retrotranscription (RT): converts RNA into complementary DNA (cDNA) using the reverse transcriptase enzyme.<br\/>&#8211; PCR: amplifies that cDNA so it can be studied.<\/p>\n\n<p>This type of PCR is essential when the target is not DNA, but RNA, such as:<\/p>\n\n<p>&#8211; RNA genome viruses, such as SARS-CoV-2, influenza, and dengue.<br\/>&#8211; Gene expression studies.<br\/>&#8211; Messenger RNA analysis in tissues.<\/p>\n\n<p>RT-PCR can be conventional or real-time (RT-qPCR), allowing RNA to be detected and quantified with very high sensitivity.<\/p>\n\n<p>In highly sensitive techniques such as PCR, qPCR, and RT-PCR, water purity defines the precision of the experiment. Salvak ultrapure water provides safety for every reaction mix, ensuring consistency in every assay.<\/p>\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Optimize your reactions with the purity you need.<\/p>\n","protected":false},"author":1,"featured_media":2292,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","format":"standard","meta":{"_joinchat":[],"footnotes":""},"etapas":[88],"class_list":["post-2290","aplicaciones","type-aplicaciones","status-publish","format-standard","has-post-thumbnail","hentry","etapas-molecular-biology-solutions"],"_links":{"self":[{"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/aplicaciones\/2290","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/aplicaciones"}],"about":[{"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/types\/aplicaciones"}],"author":[{"embeddable":true,"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/comments?post=2290"}],"version-history":[{"count":1,"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/aplicaciones\/2290\/revisions"}],"predecessor-version":[{"id":2293,"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/aplicaciones\/2290\/revisions\/2293"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/media\/2292"}],"wp:attachment":[{"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/media?parent=2290"}],"wp:term":[{"taxonomy":"etapas","embeddable":true,"href":"https:\/\/salvak.com.co\/en\/wp-json\/wp\/v2\/etapas?post=2290"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}